Variant CallingSAFE

The largest open-source medical AI skills library for OpenClaw🦞.

The instruction file, verbatim from the audited commit — this is the text the model reads, and the surface the audit's instruction layer examines. Quoted here so you can judge it without cloning anything.

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# Copyright (c) 2026 MD BABU MIA, PhD <[email protected]>
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---
name: bio-variant-calling
description: Call SNPs and indels from aligned reads using bcftools mpileup and call. Use when detecting variants from BAM files or generating VCF from alignments.
tool_type: cli
primary_tool: bcftools
measurable_outcome: Execute skill workflow successfully with valid output within 15 minutes.
allowed-tools:
  - read_file
  - run_shell_command
---

# Variant Calling

Call SNPs and indels from aligned reads using bcftools.

## Basic Workflow

```
BAM file + Reference FASTA
         |
         v
   bcftools mpileup (generate pileup)
         |
         v
   bcftools call (call variants)
         |
         v
   VCF file
```

## bcftools mpileup + call

### Basic Variant Calling
```bash
bcftools mpileup -f reference.fa input.bam | bcftools call -mv -o variants.vcf
```

### Output Compressed VCF
```bash
bcftools mpileup -f reference.fa input.bam | bcftools call -mv -Oz -o variants.vcf.gz
bcftools index variants.vcf.gz
```

### Call Specific Region
```bash
bcftools mpileup -f reference.fa -r chr1:1000000-2000000 input.bam | \
    bcftools call -mv -o region.vcf
```

### Call from Multiple BAMs
```bash
bcftools mpileup -f reference.fa sample1.bam sample2.bam sample3.bam | \
    bcftools call -mv -o variants.vcf
```

### BAM List File
```bash
# bams.txt: one BAM path per line
bcftools mpileup -f reference.fa -b bams.txt | bcftools call -mv -o variants.vcf
```

## mpileup Options

### Quality Filtering
```bash
bcftools mpileup -f reference.fa \
    -q 20 \           # Min mapping quality
    -Q 20 \           # Min base quality
    input.bam | bcftools call -mv -o variants.vcf
```

### Annotate with Read Depth
```bash
bcftools mpileup -f reference.fa -a DP,AD input.bam | bcftools call -mv -o variants.vcf
```

### Full Annotation Set
```bash
bcftools mpileup -f reference.fa \
    -a FORMAT/DP,FORMAT/AD,FORMAT/ADF,FORMAT/ADR,INFO/AD \
    input.bam | bcftools call -mv -o variants.vcf
```

### Target Regions (BED)
```bash
bcftools mpileup -f reference.fa -R targets.bed input.bam | \
    bcftools call -mv -o variants.vcf
```

### Max Depth
```bash
bcftools mpileup -f reference.fa -d 1000 input.bam | bcftools call -mv -o variants.vcf
```

## call Options

### Calling Models

| Flag | Model | Use Case |
|------|-------|----------|
| `-m` | Multiallelic caller | Default, recommended |
| `-c` | Consensus caller | Legacy, single sample |

### Output Variants Only
```bash
bcftools mpileup -f reference.fa input.bam | bcftools call -mv -o variants.vcf
# -v outputs variant sites only (not reference calls)
```

### Output All Sites
```bash
bcftools mpileup -f reference.fa input.bam | bcftools call -m -o all_sites.vcf
# Without -v, outputs all sites including reference
```

### Ploidy
```bash
# Haploid calling
bcftools mpileup -f reference.fa input.bam | bcftools call -m --ploidy 1 -o variants.vcf

# Specify ploidy file
bcftools mpileup -f reference.fa input.bam | bcftools call -m --ploidy-file ploidy.txt -o variants.vcf
```

### Prior Probability
```bash
# Adjust variant prior (default 1.1e-3)
bcftools mpileup -f reference.fa input.bam | bcftools call -m -P 0.001 -o variants.vcf
```

## Common Pipelines

### Standard SNP/Indel Calling
```bash
bcftools mpileup -Ou -f reference.fa \
    -q 20 -Q 20 \
    -a FORMAT/DP,FORMAT/AD \
    input.bam | \
bcftools call -mv -Oz -o variants.vcf.gz

bcftools index variants.vcf.gz
```

### Multi-sample Calling
```bash
bcftools mpileup -Ou -f reference.fa \
    -a FORMAT/DP,FORMAT/AD \
    sample1.bam sample2.bam sample3.bam | \
bcftools call -mv -Oz -o cohort.vcf.gz

bcftools index cohort.vcf.gz
```

### Calling with Regions
```bash
bcftools mpileup -Ou -f reference.fa \
    -R targets.bed \
    -a FORMAT/DP,FORMAT/AD \
    input.bam | \
bcftools call -mv -Oz -o targets.vcf.gz
```

### Parallel by Chromosome
```bash
for chr in chr1 chr2 chr3; do
    bcftools mpileup -Ou -f reference.fa -r "$chr" input.bam | \
        bcftools call -mv -Oz -o "${chr}.vcf.gz" &
done
wait

# Concatenate results
bcftools concat -Oz -o all.vcf.gz chr*.vcf.gz
bcftools index all.vcf.gz
```

## Annotation Tags

### INFO Tags
| Tag | Description |
|-----|-------------|
| `DP` | Total read depth |
| `AD` | Allelic depths |
| `MQ` | Mapping quality |
| `FS` | Fisher strand bias |
| `SGB` | Segregation based metric |

### FORMAT Tags
| Tag | Description |
|-----|-------------|
| `GT` | Genotype |
| `DP` | Read depth per sample |
| `AD` | Allelic depths per sample |
| `ADF` | Forward strand allelic depths |
| `ADR` | Reverse strand allelic depths |
| `GQ` | Genotype quality |
| `PL` | Phred-scaled likelihoods |

### Request Specific Annotations
```bash
bcftools mpileup -f reference.fa \
    -a FORMAT/DP,FORMAT/AD,FORMAT/SP,INFO/AD \
    input.bam | bcftools call -mv -o variants.vcf
```

## Performance Options

### Multi-threading
```bash
bcftools mpileup -f reference.fa --threads 4 input.bam | \
    bcftools call -mv --threads 4 -o variants.vcf
```

### Uncompressed BCF for Speed
```bash
bcftools mpileup -Ou -f reference.fa input.bam | bcftools call -mv -Ou | \
    bcftools filter -Oz -o filtered.vcf.gz
```

## Quick Reference

| Task | Command |
|------|---------|
| Basic calling | `bcftools mpileup -f ref.fa in.bam \| bcftools call -mv -o out.vcf` |
| With quality filter | `bcftools mpileup -f ref.fa -q 20 -Q 20 in.bam \| bcftools call -mv` |
| Region | `bcftools mpileup -f ref.fa -r chr1:1-1000 in.bam \| bcftools call -mv` |
| Multi-sample | `bcftools mpileup -f ref.fa s1.bam s2.bam \| bcftools call -mv` |
| With annotations | `bcftools mpileup -f ref.fa -a DP,AD in.bam \| bcftools call -mv` |

## Common Errors

| Error | Cause |

SAFEgrade B · trust 89/100 Nothing in the source contradicts what it says it does. Grade A is reserved for packages that have also passed the behavioural sandbox.

What the source does

Filesystem

none-observed

Network

none-observed

Shell

none-observed

Dependencies

pinned

Secrets in source

none-found

Findings (1)

Gates applied: no_behavioural_pass.